Investigation of Heterotrophic Bacteria, Legionella and Free-Living Amoeba in Cooling Tower Samples by FISH and Culture Methods

Zuhal Zeybek [1] , Nihal Dogruoz Gungor [2] , Irfan Turetgen [3]

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The microorganisms living in the cooling towers water can affect both human health through inhalation of aerosolized water as well as industrial processes. In order to analyse such man-made water systems, microbiological tests that can give results in a short time are needed. In this study, the presence of heterotrophic bacteria, Legionella bacteria and free - living amoeba, FLA, including Acanthamoeba, in cooling-tower water and biofilm samples were investigated using two different methods, fluorescent in situ hybridization (FISH), and culture. For this, a total of 40 water and biofilm samples were taken from 16 different cooling towers in Istanbul. FISH and culture analysis have revealed that the number of heterotrophic bacteria within the water and the biofilm samples was above the threshold values (>105 cell. mL-1), generally. Despite Acanthamoeba were present in all cooling tower specimens, Legionella pneumophila serogroup 1 were only detected in the biofilm of one cooling tower. According to the results of this study, both methods are recommended to be used in conjunction. Due to the the large biodiversity of FLA such as Hartmanella sp. and Naeglaria sp, there is a need for new studies utilizing FISH method for sensitive, reliable results in a short period of time.

Cooling tower water, biofilm, heterotrophic bacteria, Legionella, Acanthamoeba, free-living amoebae
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Konular Yaşam Bilimleri
Dergi Bölümü Araştırma Makaleleri
Yazarlar

Yazar: Zuhal Zeybek (Sorumlu Yazar)
E-posta: zeybek@istanbul.edu.tr
Kurum: Istanbul University, Faculty of Science, Department of Biology, Fundamental and Industrial Microbiology, İstanbul.
Ülke: Turkey


Yazar: Nihal Dogruoz Gungor
E-posta: zeybek@istanbul.edu.tr
Kurum: Istanbul University, Faculty of Science, Department of Biology, Fundamental and Industrial Microbiology, İstanbul, Turkey

Yazar: Irfan Turetgen
E-posta: zeybek@istanbul.edu.tr

Bibtex @araştırma makalesi { iufsjb371710, journal = {European Journal of Biology}, issn = {2602-2575}, address = {İstanbul Üniversitesi}, year = {}, volume = {76}, pages = {7 - 13}, doi = {10.5152/EurJBiol.2017.1702}, title = {Investigation of Heterotrophic Bacteria, Legionella and Free-Living Amoeba in Cooling Tower Samples by FISH and Culture Methods}, key = {cite}, author = {Zeybek, Zuhal and Dogruoz Gungor, Nihal and Turetgen, Irfan} }
APA Zeybek, Z , Dogruoz Gungor, N , Turetgen, I . (). Investigation of Heterotrophic Bacteria, Legionella and Free-Living Amoeba in Cooling Tower Samples by FISH and Culture Methods. European Journal of Biology, 76 (1), 7-13. DOI: 10.5152/EurJBiol.2017.1702
MLA Zeybek, Z , Dogruoz Gungor, N , Turetgen, I . "Investigation of Heterotrophic Bacteria, Legionella and Free-Living Amoeba in Cooling Tower Samples by FISH and Culture Methods". European Journal of Biology 76 (): 7-13 <http://www.dergipark.gov.tr/iufsjb/issue/33412/371710>
Chicago Zeybek, Z , Dogruoz Gungor, N , Turetgen, I . "Investigation of Heterotrophic Bacteria, Legionella and Free-Living Amoeba in Cooling Tower Samples by FISH and Culture Methods". European Journal of Biology 76 (): 7-13
RIS TY - JOUR T1 - Investigation of Heterotrophic Bacteria, Legionella and Free-Living Amoeba in Cooling Tower Samples by FISH and Culture Methods AU - Zuhal Zeybek , Nihal Dogruoz Gungor , Irfan Turetgen Y1 - 2018 PY - 2018 N1 - doi: 10.5152/EurJBiol.2017.1702 DO - 10.5152/EurJBiol.2017.1702 T2 - European Journal of Biology JF - Journal JO - JOR SP - 7 EP - 13 VL - 76 IS - 1 SN - 2602-2575-2618-6144 M3 - doi: 10.5152/EurJBiol.2017.1702 UR - http://dx.doi.org/10.5152/EurJBiol.2017.1702 Y2 - 2018 ER -
EndNote %0 European Journal of Biology Investigation of Heterotrophic Bacteria, Legionella and Free-Living Amoeba in Cooling Tower Samples by FISH and Culture Methods %A Zuhal Zeybek , Nihal Dogruoz Gungor , Irfan Turetgen %T Investigation of Heterotrophic Bacteria, Legionella and Free-Living Amoeba in Cooling Tower Samples by FISH and Culture Methods %D 2018 %J European Journal of Biology %P 2602-2575-2618-6144 %V 76 %N 1 %R doi: 10.5152/EurJBiol.2017.1702 %U 10.5152/EurJBiol.2017.1702