Yıl 2017, Cilt 76, Sayı 1, Sayfalar 14 - 19 2017-12-28

An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats

Tunc Catal [1] , Sevim Tunali [2] , Sehnaz Bolkent [3] , Refiye Yanardag [4]

118 106

The protective effects of a combination of antioxidants on histopathological changes and biochemical parameters were examined in D-galactosamine (D-GaIN)-induced hepatotoxicity in rats. Physiological saline solution was injected intraperitoneally into the control group, while D-GaIN (500 mg kg-1) was administered intraperitoneally into the experimental animals. The combination of 100 mg/kg/day ascorbic acid, 100 mg/kg/day alpha tocopherol, 15 mg/kg/day beta carotene, and 0.2 mg/kg/day sodium selenate was administered orally to intact control rats for 3 days. The same antioxidant combination was given to the D-GaIN group. Liver and blood samples were used for histopathological and biochemical assays. Liver tissues were significantly damaged by D-GaIN administration based on the histopathological findings. Serum aspartate and alanine transaminase, gamma glutamyl transpeptidase, alkaline phosphatase, lactate dehydrogenase, sialic acid, and uric acid levels increased. Serum and liver glutathione levels decreased, and serum superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase, catalase activities as well as lipid peroxidation values increased in the D-GaIN group. In contrast, administration of the antioxidants reversed the histopathological and biochemical changes in the liver of animals administered D-GaIN. In conclusion, the administration of a combination of antioxidants suppressed histopathological changes and biochemical parameters in rats given D-GaIN.

Vitamin C, β-carotene, D-galactosamine, sodium selenate, vitamin E, liver damage
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Konular Yaşam Bilimleri
Dergi Bölümü Araştırma Makaleleri
Yazarlar

Yazar: Tunc Catal
E-posta: stunali@istanbul.edu.tr
Kurum: Uskudar University, Faculty of Engineering and Natural Sciences, Department of Molecular Biology, Istanbul, Turkey

Yazar: Sevim Tunali (Sorumlu Yazar)
E-posta: stunali@istanbul.edu.tr
Kurum: Istanbul University, Faculty of Engineering, Department of Chemistry, Istanbul, Turkey

Yazar: Sehnaz Bolkent
E-posta: stunali@istanbul.edu.tr
Kurum: Istanbul University, Faculty of Science, Department of Biology, Istanbul, Turkey

Yazar: Refiye Yanardag
E-posta: stunali@istanbul.edu.tr
Kurum: Istanbul University, Faculty of Engineering, Department of Chemistry, Istanbul, Turkey

Bibtex @araştırma makalesi { iufsjb371711, journal = {European Journal of Biology}, issn = {2602-2575}, address = {İstanbul Üniversitesi}, year = {2017}, volume = {76}, pages = {14 - 19}, doi = {10.5152/EurJBiol.2017.1703}, title = {An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats}, key = {cite}, author = {Catal, Tunc and Tunali, Sevim and Bolkent, Sehnaz and Yanardag, Refiye} }
APA Catal, T , Tunali, S , Bolkent, S , Yanardag, R . (2017). An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats. European Journal of Biology, 76 (1), 14-19. DOI: 10.5152/EurJBiol.2017.1703
MLA Catal, T , Tunali, S , Bolkent, S , Yanardag, R . "An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats". European Journal of Biology 76 (2017): 14-19 <http://www.dergipark.gov.tr/iufsjb/issue/33412/371711>
Chicago Catal, T , Tunali, S , Bolkent, S , Yanardag, R . "An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats". European Journal of Biology 76 (2017): 14-19
RIS TY - JOUR T1 - An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats AU - Tunc Catal , Sevim Tunali , Sehnaz Bolkent , Refiye Yanardag Y1 - 2017 PY - 2017 N1 - doi: 10.5152/EurJBiol.2017.1703 DO - 10.5152/EurJBiol.2017.1703 T2 - European Journal of Biology JF - Journal JO - JOR SP - 14 EP - 19 VL - 76 IS - 1 SN - 2602-2575-2618-6144 M3 - doi: 10.5152/EurJBiol.2017.1703 UR - http://dx.doi.org/10.5152/EurJBiol.2017.1703 Y2 - 2018 ER -
EndNote %0 European Journal of Biology An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats %A Tunc Catal , Sevim Tunali , Sehnaz Bolkent , Refiye Yanardag %T An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats %D 2017 %J European Journal of Biology %P 2602-2575-2618-6144 %V 76 %N 1 %R doi: 10.5152/EurJBiol.2017.1703 %U 10.5152/EurJBiol.2017.1703