Yıl 2017, Cilt 76, Sayı 1, Sayfalar 26 - 30 2017-12-27

Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos

Unsal Veli Ustundag [1] , Ismail Unal [2] , Perihan Seda Ates [3] , Ahmet Ata Alturfan [4] , Turkan Yigitbasi [5] , Ebru Emekli Alturfan [6]

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Di-(2-ethylhexyl)-phthalate (DEHP) is used in polyethylene terephthalate plastics and bisphenol-A (BPA) is used to make polycarbonate plastics. The health-concerning effects of DEHP and BPA indicate their potential toxic effects. The Wnt/β-catenin signaling pathway is necessary for embryonic development. The c-MYC proto-oncogene (MYC) has been identified as a Wnt/β-catenin target gene, and MYC is a transcription factor that regulates the expression of many gene products related to cell proliferation, growth, differentiation, and apoptosis. Activation and overexpression of the MYC gene has been reported in many human cancers and during tumorigenesis. The aim of this study was to investigate the relationship between oxidant-antioxidant balance and c-myc in BPA- and DEHP-exposed zebrafish embryos. The real-time-polymerase chain reaction was used to determine c-myc expression. Lipid peroxidation (LPO), nitric oxide (NO) levels, and superoxide dismutase (SOD) and glutathione-S-transferase (GST) activities were determined in homogenates prepared from pooled embryo samples. Increased expression of c-myc and decreased GST activity were observed in the BPA and DEHP groups. NO levels increased and SOD activity decreased in the BPA group, whereas LPO increased in the DEHP group. Disruption of oxidant-antioxidant balance in DEHP- and BPA-exposed zebrafish embryos was associated with increased c-myc expression and may be an important mechanism for the toxic effects of these chemicals. 

Di(2-ethylhexyl) phthalate, bisphenol A, oxidant-antioxidant balance, c-myc, zebrafish embryo
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Konular Fen
Dergi Bölümü Araştırma Makaleleri
Yazarlar

Yazar: Unsal Veli Ustundag
Kurum: Istanbul Medipol University, School of Medicine, Department of Biochemistry, Istanbul, Turkey

Yazar: Ismail Unal
Kurum: Marmara University, Faculty of Dentistry, Department of Biochemistry, Istanbul, Turkey

Yazar: Perihan Seda Ates
Kurum: Marmara University, Faculty of Dentistry, Department of Biochemistry, Istanbul, Turkey

Yazar: Ahmet Ata Alturfan
Kurum: Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey

Yazar: Turkan Yigitbasi
Kurum: Istanbul Medipol University, School of Medicine, Department of Biochemistry, Istanbul, Turkey

Yazar: Ebru Emekli Alturfan (Sorumlu Yazar)
Kurum: Marmara University, Faculty of Dentistry, Department of Biochemistry, Istanbul, Turkey

Bibtex @araştırma makalesi { iufsjb371714, journal = {European Journal of Biology}, issn = {2602-2575}, eissn = {2618-6144}, address = {İstanbul Üniversitesi}, year = {2017}, volume = {76}, pages = {26 - 30}, doi = {10.5152/EurJBiol.2017.1705}, title = {Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos}, key = {cite}, author = {Ustundag, Unsal Veli and Yigitbasi, Turkan and Ates, Perihan Seda and Unal, Ismail and Emekli Alturfan, Ebru and Alturfan, Ahmet Ata} }
APA Ustundag, U , Unal, I , Ates, P , Alturfan, A , Yigitbasi, T , Emekli Alturfan, E . (2017). Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos. European Journal of Biology, 76 (1), 26-30. DOI: 10.5152/EurJBiol.2017.1705
MLA Ustundag, U , Unal, I , Ates, P , Alturfan, A , Yigitbasi, T , Emekli Alturfan, E . "Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos". European Journal of Biology 76 (2017): 26-30 <http://www.dergipark.gov.tr/iufsjb/issue/33412/371714>
Chicago Ustundag, U , Unal, I , Ates, P , Alturfan, A , Yigitbasi, T , Emekli Alturfan, E . "Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos". European Journal of Biology 76 (2017): 26-30
RIS TY - JOUR T1 - Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos AU - Unsal Veli Ustundag , Ismail Unal , Perihan Seda Ates , Ahmet Ata Alturfan , Turkan Yigitbasi , Ebru Emekli Alturfan Y1 - 2017 PY - 2017 N1 - doi: 10.5152/EurJBiol.2017.1705 DO - 10.5152/EurJBiol.2017.1705 T2 - European Journal of Biology JF - Journal JO - JOR SP - 26 EP - 30 VL - 76 IS - 1 SN - 2602-2575-2618-6144 M3 - doi: 10.5152/EurJBiol.2017.1705 UR - http://dx.doi.org/10.5152/EurJBiol.2017.1705 Y2 - 2018 ER -
EndNote %0 European Journal of Biology Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos %A Unsal Veli Ustundag , Ismail Unal , Perihan Seda Ates , Ahmet Ata Alturfan , Turkan Yigitbasi , Ebru Emekli Alturfan %T Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos %D 2017 %J European Journal of Biology %P 2602-2575-2618-6144 %V 76 %N 1 %R doi: 10.5152/EurJBiol.2017.1705 %U 10.5152/EurJBiol.2017.1705
ISNAD Ustundag, Unsal Veli , Unal, Ismail , Ates, Perihan Seda , Alturfan, Ahmet Ata , Yigitbasi, Turkan , Emekli Alturfan, Ebru . "Oxidant-Antioxidant Status and c-myc Expression in BPA- and DEHP-Exposed Zebrafish Embryos". European Journal of Biology 76 / 1 (Aralık 2017): 26-30. http://dx.doi.org/10.5152/EurJBiol.2017.1705