Cilt 4, Sayı 4, Sayfalar 385 - 392 2017-10-26

Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method

Touseef HUSSAIN [1] , Bir Pal SINGH [2] , Firoz ANWAR [3]

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Oomycete pathogens of the genus Phytophthora are the most destructive plant pathogens known. They spread mainly through the movement of infested soil, water and infected plants and plant material. Especially damaging as a source of inoculum are those plants/seeds that are infected but do not show signs of symptoms either because the disease has not yet progressed to the stage where symptoms are evident, or due to suppression of symptom development by the use of fungicides. In our indirect ELISA method, P.infestans exhibited strong positive reaction with sporangia (2.256), mycelium (1.256) as well as oospores (2.286) whereas no reaction with other fungal pathogens of potato. P.infestans was detected by indirect ELISA in potato leaves (1.212) and tubers (1.201). Our study was to confirm the detection of P.infestans irrespective of inoculum present in the host tissues (0.435), by which planting material by quarantine dept., horticultural dept. from one state to another. It could be used as reliable routine diagnostic test to replace current identification and isolation, detection methods.

Phytophthora infestans,oospores,potato,ELISA
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Konular
Dergi Bölümü Makaleler
Yazarlar

Yazar: Touseef HUSSAIN
E-posta: hussaintouseef@yahoo.co.in
Ülke: India


Yazar: Bir Pal SINGH
E-posta: hussaintouseef@yahoo.co.in
Ülke: India


Yazar: Firoz ANWAR
E-posta: hussaintouseef@yahoo.co.in
Ülke: Saudi Arabia


Bibtex @araştırma makalesi { turkjans346663, journal = {Türk Tarım ve Doğa Bilimleri Dergisi-Turkish Journal of Agricultural and Natural Sciences}, issn = {2148-3647}, address = {Mevlüt AKÇURA}, year = {2017}, volume = {4}, pages = {385 - 392}, doi = {}, title = {Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method}, language = {tr}, key = {cite}, author = {HUSSAIN, Touseef and ANWAR, Firoz and SINGH, Bir Pal} }
APA HUSSAIN, T , SINGH, B , ANWAR, F . (2017). Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method. Türk Tarım ve Doğa Bilimleri Dergisi-Turkish Journal of Agricultural and Natural Sciences, 4 (4), 385-392. Retrieved from http://www.dergipark.gov.tr/turkjans/issue/31628/346663
MLA HUSSAIN, T , SINGH, B , ANWAR, F . "Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method". Türk Tarım ve Doğa Bilimleri Dergisi-Turkish Journal of Agricultural and Natural Sciences 4 (2017): 385-392 <http://www.dergipark.gov.tr/turkjans/issue/31628/346663>
Chicago HUSSAIN, T , SINGH, B , ANWAR, F . "Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method". Türk Tarım ve Doğa Bilimleri Dergisi-Turkish Journal of Agricultural and Natural Sciences 4 (2017): 385-392
RIS TY - JOUR T1 - Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method AU - Touseef HUSSAIN , Bir Pal SINGH , Firoz ANWAR Y1 - 2017 PY - 2017 N1 - DO - T2 - Türk Tarım ve Doğa Bilimleri Dergisi-Turkish Journal of Agricultural and Natural Sciences JF - Journal JO - JOR SP - 385 EP - 392 VL - 4 IS - 4 SN - 2148-3647- M3 - UR - Y2 - 2017 ER -
EndNote %0 Türk Tarım ve Doğa Bilimleri Dergisi-Turkish Journal of Agricultural and Natural Sciences Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method %A Touseef HUSSAIN , Bir Pal SINGH , Firoz ANWAR %T Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method %D 2017 %J Türk Tarım ve Doğa Bilimleri Dergisi-Turkish Journal of Agricultural and Natural Sciences %P 2148-3647- %V 4 %N 4 %R %U